The Merchant lab seeks a post-doctoral fellow with an interest in hematopoiesis, stem cell biology, and/or myeloproliferative diseases to join an NIH funded project examining the role of Hedgehog signaling in the bone marrow microenvironment.Studies in the lab make use of transgenic mouse models and primary patient samples.There will be additional opportunities to study patient samples from patients treated with hedgehog inhibitors.Studies of the bone marrow microenvironment will be enabled but the use of imaging mass cytometry/CyTOF.Imaging mass cytometry allows for highly multiplexed (35+ markers) imaging of immune and tumor cells, with spatial information and subcellular resolution making it the ideal technology for studying stem cells and immune cells in their cellular niches within the bone marrow.Imaging mass cytometry can be applied to all tissue types including cell lines, organoids, frozen section and FFPE fixed archival tissue.
Primary Duties and Responsibilities:
Perform experiments with transgenic mice including breeding, genotyping, blood sampling and bone marrow harvesting.
Apply standard molecular and cellular biology methods (RT-PCR, Western blotting, cell culture and transduction, expression profiling) to profile the hedgehog signaling pathway.
Use imaging mass cytometry to study the bone marrow microenvironment and stromal cell changes in animal models of myelofibrosis and in-patient samples.
Will assist in preparation of grant proposals but is not responsible for generating grant funds.Will be encouraged to apply for fellowships as part of career development
Will participate in publications and presentations as author or co-author.
May train and supervise laboratory assistants and/or graduate students
Designs and performs experiments. Will keep appropriate experimental records and documentation and analyze the results with the Principal Investigator.
May develop, adapt, and implement new research techniques and protocols.
Analyzes, interprets, summarizes and compiles data.
Performs routine and complex laboratory procedures throughout training period.
Since its beginning in 1902, Cedars-Sinai has evolved to meet the healthcare needs of one of the most diverse regions in the nation, continually setting new standards in quality and innovation in patient care, research, teaching and community service. Today, Cedars-Sinai is widely known for its national leadership in transforming healthcare for the benefit of patients.Cedars-Sinai is one of the largest nonprofit academic medical centers in the U.S. with 886 licensed beds, 2,100 physicians, 2,800 nurses and thousands of other healthcare professionals and staff. Clinical programs range from primary care for preventing, diagnosing and treating common conditions to specialized treatments for rare, complex and advanced illnesses. In addition, Cedars-Sinai serves the community through its Medical Network, which includes the highly rated Cedars-Sinai Medical Group and Cedars-Sinai Health Associates. Cedars-Sinai has consistently been named one of America’s Best Hospitals by U.S. News & World Report, has received the National Research Corporation’s Consumer Choice Award 18 years in a row for providing the highest quality medical care in Los Angeles, and has the longest running Magnet desig...nation for nursing excellence in California. Cedars-Sinai is a leader in the clinical care and research of heart disease, cancer and brain disorders, among other areas. Pioneering research achievements include using cardiac stem cells to repair damaged hearts, developing minimally invasive surgical techniques and discovering new types of drugs to target cancer more precisely. Cedars-Sinai also impacts the future of healthcare through education programs that encompass everything from highly competitive medical residency and fellowship programs to a biomedical science and translational medicine PhD program, advanced training for nurses and educational opportunities for allied health professionals. Most notably, Cedars-Sinai demonstrates a longstanding commitment to strengthening the Los Angeles community through wide-ranging programs that improve the health of its most vulnerable residents.